Lab diagnosis of syphilis:
Principle:Labrotaroy diagnosis of secondary syphilis of direct demonstration of treponema by M/E and serological tests.
- a) Tissue fluids from the lesion particularly from the primary chance or secondary rash.
- b) Serum for serology.
- c) CSF(Cerebro spinal fluid).
[A]Microscopically examination:Dark ground Illumination(DGI) using oil immersion lense.
Findings:Bright red colour and cork screw movement of treponema palladium can be seen.
[B]Immunofluroscence test:Tissue fluid is smeared on a slide,air dried fixed and stained by fluroscen labeled antitreponema serum.
(A)Non Treponemal antibody test/Non specific antibody test:Reagin test:Detection of regain
a) VDRL(Veneral disease reasearch lab test)
b) RPR(Rapid plasma reagen test)
c) CFT(Compliment fixation test)
(B)Treponemal antibody test/Specific antibody test:
a) TPHA(Treponama pallidum haemagglutination test):The principle of test in RBC (absorbed treponemast patients serium)
b) TPA(Treponama pallidum antibody test)
c) FTA-ABS(Fluorosent treponemal antibody absorption test):Here patients serum should be absorbed by sonicated Reitters stain. Killed T pallidum+Absorbed patient serum+Lable antihuman gamma globulin.
d) TPI(Treponema pallidum immobilization test):This tests requires the highly infective strain.Treponema pallidum of the Nicoles strain.
e) Compliment fixation test:RP(CFT):Reitters protein compliment fixation test.This test is more specific than the STS(Standered test for syphilis)
(C)ELISA(Enzyme linked immuno sorbant assay):If newly introduced techniques that is introduced this test is very simply to perform and is equivalent to the FTA(ABC)
a) Routine examination.
b) Langes test.